CertiChem offers BG1Luc assays to assess the estrogenic and anti-estrogenic activities for chemicals and chemical mixtures. BG1Luc agonist assay is a luciferase reporter gene assay that has an increased amount of luciferase upon the binding of ER agonists to ER and reduce the agonist reference agonist (e.g., 17-estradiaol)-induced luciferase expression upon the binding of ER antagonists to ER. Both BG1Luc ER agonist and antagonist were validated by ICCVAM in a manual 96-well format and then were successfully converted to a robotic assay by CCi (Stoner, 2015). Our BG1Luc assays are customized to meet the specific needs of our clients.
The advantages of our in vitro BG1Luc assays for detecting estrogenic and anti-estrogenic activities include:
(1) High accuracy, sensitivity and specificity.
(2) Ability to analyze mixtures of known or unknown chemicals.
(3) Low cost.
(4) Ability to analyze small amounts of suspect chemicals or chemical mixtures.
(5) In vitro assays can reduce animal use in compliance with protocols of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM).
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